pcDNA3.1_hE2F7mg_PP7-tag
(Plasmid #73078)

• Purpose: human E2F7 minigene (exons 11,12,13/introns cassette) with PP7-tag on 5'-end
• Depositing Lab: Stefan Stamm
• Publication: Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8.

Backbone

• Vector backbone: pcDNA3.1V5hisTOPO
• Total vector size (bp): 9738
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E2F7
• Species: H. sapiens (human)
• Insert Size (bp): 4178
• Mutation: partial gene, exons 11,12,13 spaced by introns
• Entrez Gene: E2F7
• Tag / Fusion Protein:
  • 5'-PP7-tag RNA

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: TGGAAACCCTTAGACGTCAAACCCTCAGATTCCACAG
• 3′ sequencing primer: TATAAACTCCTTAAAGCTTAAGGGCAATTCCACCACAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry