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Addgene

pcDNA3.1_hE2F7mg
(Plasmid #73073)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73073 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1V5hisTOPO
  • Total vector size (bp) 9701
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    E2F7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4178
  • Mutation
    partial gene, exons 11,12,13 spaced by introns
  • Entrez Gene
    E2F7
  • Promoter CMV

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer AAACCCTCAGATTCCACAG
  • 3′ sequencing primer ACATCCGGGACTCTCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_hE2F7mg was a gift from Stefan Stamm (Addgene plasmid # 73073 ; http://n2t.net/addgene:73073 ; RRID:Addgene_73073)

  • For your References section:

    Dual function of C/D box small nucleolar RNAs in rRNA modification and alternative pre-mRNA splicing. Falaleeva M, Pages A, Matuszek Z, Hidmi S, Agranat-Tamir L, Korotkov K, Nevo Y, Eyras E, Sperling R, Stamm S. Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):E1625-34. doi: 10.1073/pnas.1519292113. Epub 2016 Mar 8. 10.1073/pnas.1519292113 PubMed 26957605
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