pCMV-BE3 Citations (70)

Originally described in: Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage.
Komor AC, Kim YB, Packer MS, Zuris JA, Liu DR Nature. 2016 Apr 20. doi: 10.1038/nature17946. PubMed Journal

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Articles Citing pCMV-BE3

Articles
Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System. Li J, Sun Y, Du J, Zhao Y, Xia L. Mol Plant. 2016 Dec 8. pii: S1674-2052(16)30298-2. doi: 10.1016/j.molp.2016.12.001. PubMed
Highly efficient RNA-guided base editing in mouse embryos. Kim K, Ryu SM, Kim ST, Baek G, Kim D, Lim K, Chung E, Kim S, Kim JS. Nat Biotechnol. 2017 Feb 27. doi: 10.1038/nbt.3816. PubMed Associated Plasmids pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Genome-wide target specificities of CRISPR RNA-guided programmable deaminases. Kim D, Lim K, Kim ST, Yoon SH, Kim K, Ryu SM, Kim JS. Nat Biotechnol. 2017 May;35(5):475-480. doi: 10.1038/nbt.3852. Epub 2017 Apr 10. PubMed Associated Plasmids pET28b-BE3∆UGI
CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations. Kuscu C, Parlak M, Tufan T, Yang J, Szlachta K, Wei X, Mammadov R, Adli M. Nat Methods. 2017 Jul;14(7):710-712. doi: 10.1038/nmeth.4327. Epub 2017 Jun 5. PubMed
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Zhang Y, Qin W, Lu X, Xu J, Huang H, Bai H, Li S, Lin S. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. PubMed Associated Plasmids pCMV-BE-zCas9 pCMV-BE-VQRCas9
In Vivo Base Editing of PCSK9 (Proprotein Convertase Subtilisin/Kexin Type 9) as a Therapeutic Alternative to Genome Editing. Chadwick AC, Wang X, Musunuru K. Arterioscler Thromb Vasc Biol. 2017 Sep;37(9):1741-1747. doi: 10.1161/ATVBAHA.117.309881. Epub 2017 Jul 27. PubMed
CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons. Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, Ciccia A. Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008. PubMed Associated Plasmids B52 (empty plasmid backbone to express 2 sgRNAs) B52 + SPRTN sgSTOP B52 + FANCM sgSTOP B52 + PARP4 sgSTOP B52 + CHEK2 sgSTOP More ...
APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks. Lei L, Chen H, Xue W, Yang B, Hu B, Wei J, Wang L, Cui Y, Li W, Wang J, Yan L, Shang W, Gao J, Sha J, Zhuang M, Huang X, Shen B, Yang L, Chen J. Nat Struct Mol Biol. 2018 Jan;25(1):45-52. doi: 10.1038/s41594-017-0004-6. Epub 2017 Dec 11. PubMed
Heterozygous IDH1(R132H/WT) created by "single base editing" inhibits human astroglial cell growth by downregulating YAP. Wei S, Wang J, Oyinlade O, Ma D, Wang S, Kratz L, Lal B, Xu Q, Liu S, Shah SR, Zhang H, Li Y, Quinones-Hinojosa A, Zhu H, Huang ZY, Cheng L, Qian J, Xia S. Oncogene. 2018 Sep;37(38):5160-5174. doi: 10.1038/s41388-018-0334-9. Epub 2018 May 30. PubMed
BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Jiang W, Feng S, Huang S, Yu W, Li G, Yang G, Liu Y, Zhang Y, Zhang L, Hou Y, Chen J, Chen J, Huang X. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4. PubMed Associated Plasmids pST1374-GCN4-D10A pST1374-GCN4-dCas9 pST1374-GCN4-H840A pST1374-D10A-GCN4 pST1374-dCas9-GCN4 More ...
Highly efficient RNA-guided base editing in rabbit. Liu Z, Chen M, Chen S, Deng J, Song Y, Lai L, Li Z. Nat Commun. 2018 Jul 13;9(1):2717. doi: 10.1038/s41467-018-05232-2. PubMed
Introduction of pathogenic mutations into the mouse Psen1 gene by Base Editor and Target-AID. Sasaguri H, Nagata K, Sekiguchi M, Fujioka R, Matsuba Y, Hashimoto S, Sato K, Kurup D, Yokota T, Saido TC. Nat Commun. 2018 Jul 24;9(1):2892. doi: 10.1038/s41467-018-05262-w. PubMed
Directed evolution of CRISPR-Cas9 to increase its specificity. Lee JK, Jeong E, Lee J, Jung M, Shin E, Kim YH, Lee K, Jung I, Kim D, Kim S, Kim JS. Nat Commun. 2018 Aug 6;9(1):3048. doi: 10.1038/s41467-018-05477-x. PubMed Associated Plasmids p3s-Sniper-Cas9
CRISPR-SKIP: programmable gene splicing with single base editors. Gapinske M, Luu A, Winter J, Woods WS, Kostan KA, Shiva N, Song JS, Perez-Pinera P. Genome Biol. 2018 Aug 15;19(1):107. doi: 10.1186/s13059-018-1482-5. PubMed
Correction of the Marfan Syndrome Pathogenic FBN1 Mutation by Base Editing in Human Cells and Heterozygous Embryos. Zeng Y, Li J, Li G, Huang S, Yu W, Zhang Y, Chen D, Chen J, Liu J, Huang X. Mol Ther. 2018 Nov 7;26(11):2631-2637. doi: 10.1016/j.ymthe.2018.08.007. Epub 2018 Aug 14. PubMed
Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion. Zheng K, Wang Y, Li N, Jiang FF, Wu CX, Liu F, Chen HC, Liu ZF. Commun Biol. 2018 Apr 19;1:32. doi: 10.1038/s42003-018-0035-5. eCollection 2018. PubMed
Simultaneous zygotic inactivation of multiple genes in mouse through CRISPR/Cas9-mediated base editing. Zhang H, Pan H, Zhou C, Wei Y, Ying W, Li S, Wang G, Li C, Ren Y, Li G, Ding X, Sun Y, Li GL, Song L, Li Y, Yang H, Liu Z. Development. 2018 Oct 17;145(20). pii: dev.168906. doi: 10.1242/dev.168906. PubMed
CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. Li Q, Li Y, Yang S, Huang S, Yan M, Ding Y, Tang W, Lou X, Yin Q, Sun Z, Lu L, Shi H, Wang H, Chen Y, Li J. Nat Cell Biol. 2018 Oct 1. pii: 10.1038/s41556-018-0202-4. doi: 10.1038/s41556-018-0202-4. PubMed Associated Plasmids PB-BE3-NLS PB-BE3-2NLS PB-BE3-3NLS
In utero CRISPR-mediated therapeutic editing of metabolic genes. Rossidis AC, Stratigis JD, Chadwick AC, Hartman HA, Ahn NJ, Li H, Singh K, Coons BE, Li L, Lv W, Zoltick PW, Alapati D, Zacharias W, Jain R, Morrisey EE, Musunuru K, Peranteau WH. Nat Med. 2018 Oct;24(10):1513-1518. doi: 10.1038/s41591-018-0184-6. Epub 2018 Oct 8. PubMed
Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng FL. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. PubMed Associated Plasmids pLL1 sgRNA(MS2)-MS2-AIDmono pLL2 sgRNA(MS2)-MS2-AID3c pLL3 sgRNA(MS2)-MS2-AID3f pLL4 sgRNA(MS2)-MS2-hA3a pLL5 sgRNA(MS2)-MS2-hA3a D131A More ...
Minimal PAM specificity of a highly similar SpCas9 ortholog. Chatterjee P, Jakimo N, Jacobson JM. Sci Adv. 2018 Oct 24;4(10):eaau0766. doi: 10.1126/sciadv.aau0766. eCollection 2018 Oct. PubMed Associated Plasmids pScCas9
CRISPR-Cas9(D10A) nickase-assisted base editing in the solvent producer Clostridium beijerinckii. Li Q, Seys FM, Minton NP, Yang J, Jiang Y, Jiang W, Yang S. Biotechnol Bioeng. 2019 Feb 10. doi: 10.1002/bit.26949. PubMed Associated Plasmids pCBEclos-cbei2385-g2-opt
Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements. Hwang B, Lee W, Yum SY, Jeon Y, Cho N, Jang G, Bang D. Nat Commun. 2019 Mar 15;10(1):1234. doi: 10.1038/s41467-019-09203-z. PubMed
Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors. Grunewald J, Zhou R, Garcia SP, Iyer S, Lareau CA, Aryee MJ, Joung JK. Nature. 2019 Apr 17. pii: 10.1038/s41586-019-1161-z. doi: 10.1038/s41586-019-1161-z. PubMed Associated Plasmids nCas9-UGI-NLS-P2A-EGFP (pJUL1001) BE3-P2A-EGFP (pJUL977) BE3(E63Q)-P2A-EGFP (pRZ189) SECURE BE3(R33A)-P2A-EGFP (pJUL1085) SECURE BE3(R33A/K34A)-P2A-EGFP (pJUL1410) More ...
Screening of CRISPR/Cas base editors to target the AMD high-risk Y402H complement factor H variant. Tran MTN, Khalid MKNM, Pebay A, Cook AL, Liang HH, Wong RCB, Craig JE, Liu GS, Hung SS, Hewitt AW. Mol Vis. 2019 Mar 16;25:174-182. eCollection 2019. PubMed
EditR: A Method to Quantify Base Editing from Sanger Sequencing. Kluesner MG, Nedveck DA, Lahr WS, Garbe JR, Abrahante JE, Webber BR, Moriarity BS. CRISPR J. 2018 Jun;1:239-250. doi: 10.1089/crispr.2018.0014. PubMed
Improved base editor for efficient editing in GC contexts in rabbits with an optimized AID-Cas9 fusion. Liu Z, Shan H, Chen S, Chen M, Zhang Q, Lai L, Li Z. FASEB J. 2019 Aug;33(8):9210-9219. doi: 10.1096/fj.201900476RR. Epub 2019 May 9. PubMed
Base pair editing in goat: nonsense codon introgression into FGF5 results in longer hair. Li G, Zhou S, Li C, Cai B, Yu H, Ma B, Huang Y, Ding Y, Liu Y, Ding Q, He C, Zhou J, Wang Y, Zhou G, Li Y, Yan Y, Hua J, Petersen B, Jiang Y, Sonstegard T, Huang X, Chen Y, Wang X. FEBS J. 2019 Jul 5. doi: 10.1111/febs.14983. PubMed
Expanding C-T base editing toolkit with diversified cytidine deaminases. Cheng TL, Li S, Yuan B, Wang X, Zhou W, Qiu Z. Nat Commun. 2019 Aug 9;10(1):3612. doi: 10.1038/s41467-019-11562-6. PubMed Associated Plasmids N-4-BE N-8-BE C-1-BE C-6-BE C-7-BE More ...
A CRISPR-based base-editing screen for the functional assessment of BRCA1 variants. Kweon J, Jang AH, Shin HR, See JE, Lee W, Lee JW, Chang S, Kim K, Kim Y. Oncogene. 2020 Jan;39(1):30-35. doi: 10.1038/s41388-019-0968-2. Epub 2019 Aug 29. PubMed
Adenine base editors catalyze cytosine conversions in human cells. Kim HS, Jeong YK, Hur JK, Kim JS, Bae S. Nat Biotechnol. 2019 Oct;37(10):1145-1148. doi: 10.1038/s41587-019-0254-4. Epub 2019 Sep 23. PubMed
Targeted exon skipping with AAV-mediated split adenine base editors. Winter J, Luu A, Gapinske M, Manandhar S, Shirguppe S, Woods WS, Song JS, Perez-Pinera P. Cell Discov. 2019 Aug 20;5:41. doi: 10.1038/s41421-019-0109-7. eCollection 2019. PubMed
Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors. Webber BR, Lonetree CL, Kluesner MG, Johnson MJ, Pomeroy EJ, Diers MD, Lahr WS, Draper GM, Slipek NJ, Smeester BA, Lovendahl KN, McElroy AN, Gordon WR, Osborn MJ, Moriarity BS. Nat Commun. 2019 Nov 19;10(1):5222. doi: 10.1038/s41467-019-13007-6. PubMed
Construction and optimization of a base editor based on the MS2 system. Zhang S, Feng S, Jiang W, Huang X, Chen J. Animal Model Exp Med. 2019 Sep 5;2(3):185-190. doi: 10.1002/ame2.12080. eCollection 2019 Sep. PubMed
Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor. Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, Marshall JL, Chen F. Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8. PubMed Associated Plasmids pUC19 - T7 pro - IRES - EGFP pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato pLX - T7pro - EBFP
Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures. Billon P, Nambiar TS, Hayward SB, Zafra MP, Schatoff EM, Oshima K, Dunbar A, Breinig M, Park YC, Ryu HS, Tschaharganeh DF, Levine RL, Baer R, Ferrando A, Dow LE, Ciccia A. Cell Rep. 2020 Mar 10;30(10):3280-3295.e6. doi: 10.1016/j.celrep.2020.02.068. PubMed Associated Plasmids sgRNA BRCA2 V572I sgRNA BRCA2 V2102I sgRNA BRCA2 E2772K sgRNA BRCA2 R2842C sgRNA BRCA2 S3070F More ...
Single-cell analysis of a mutant library generated using CRISPR-guided deaminase in human melanoma cells. Jun S, Lim H, Chun H, Lee JH, Bang D. Commun Biol. 2020 Apr 2;3(1):154. doi: 10.1038/s42003-020-0888-2. PubMed
Enabling large-scale genome editing at repetitive elements by reducing DNA nicking. Smith CJ, Castanon O, Said K, Volf V, Khoshakhlagh P, Hornick A, Ferreira R, Wu CT, Guell M, Garg S, Ng AHM, Myllykallio H, Church GM. Nucleic Acids Res. 2020 May 21;48(9):5183-5195. doi: 10.1093/nar/gkaa239. PubMed Associated Plasmids dABE dCBE4-gam pSB700 HL1gR4
Comparison of gene disruption induced by cytosine base editing-mediated iSTOP with CRISPR/Cas9-mediated frameshift. Dang L, Li G, Wang X, Huang S, Zhang Y, Miao Y, Zeng L, Cui S, Huang X. Cell Prolif. 2020 May;53(5):e12820. doi: 10.1111/cpr.12820. Epub 2020 Apr 29. PubMed
Multiplex precise base editing in cynomolgus monkeys. Zhang W, Aida T, Del Rosario RCH, Wilde JJ, Ding C, Zhang X, Baloch Z, Huang Y, Tang Y, Li D, Lu H, Zhou Y, Jiang M, Xu D, Fang Z, Zheng Z, Huang Q, Feng G, Yang S. Nat Commun. 2020 May 11;11(1):2325. doi: 10.1038/s41467-020-16173-0. PubMed
Dual base editor catalyzes both cytosine and adenine base conversions in human cells. Zhang X, Zhu B, Chen L, Xie L, Yu W, Wang Y, Li L, Yin S, Yang L, Hu H, Han H, Li Y, Wang L, Chen G, Ma X, Geng H, Huang W, Pang X, Yang Z, Wu Y, Siwko S, Kurita R, Nakamura Y, Yang L, Liu M, Li D. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0527-y. doi: 10.1038/s41587-020-0527-y. PubMed Associated Plasmids A&C-BEmax AID-BE4max-P2A-GFP Lenti ABE7.10-N-AIDmax Lenti AID-BEmax Lenti U6-HBG site1-EF1alpha-UGI-P2A-GFP
Base editors for simultaneous introduction of C-to-T and A-to-G mutations. Sakata RC, Ishiguro S, Mori H, Tanaka M, Tatsuno K, Ueda H, Yamamoto S, Seki M, Masuyama N, Nishida K, Nishimasu H, Arakawa K, Kondo A, Nureki O, Tomita M, Aburatani H, Yachie N. Nat Biotechnol. 2020 Jun 1. pii: 10.1038/s41587-020-0509-0. doi: 10.1038/s41587-020-0509-0. PubMed Associated Plasmids pRS0045 pRS0035 pLV-SI-121 pLV-SI-112 pSI-535 More ...
Cas12a Base Editors Induce Efficient and Specific Editing with Low DNA Damage Response. Wang X, Ding C, Yu W, Wang Y, He S, Yang B, Xiong YC, Wei J, Li J, Liang J, Lu Z, Zhu W, Wu J, Zhou Z, Huang X, Liu Z, Yang L, Chen J. Cell Rep. 2020 Jun 2;31(9):107723. doi: 10.1016/j.celrep.2020.107723. PubMed Associated Plasmids pCMV-BEACON1 pCMV-BEACON2
AcrIIA5 Suppresses Base Editors and Reduces Their Off-Target Effects. Liang M, Sui T, Liu Z, Chen M, Liu H, Shan H, Lai L, Li Z. Cells. 2020 Jul 27;9(8). pii: cells9081786. doi: 10.3390/cells9081786. PubMed
A positive, growth-based PAM screen identifies noncanonical motifs recognized by the S. pyogenes Cas9. Collias D, Leenay RT, Slotkowski RA, Zuo Z, Collins SP, McGirr BA, Liu J, Beisel CL. Sci Adv. 2020 Jul 15;6(29):eabb4054. doi: 10.1126/sciadv.abb4054. eCollection 2020 Jul. PubMed
sgBE: a structure-guided design of sgRNA architecture specifies base editing window and enables simultaneous conversion of cytosine and adenosine. Wang Y, Zhou L, Tao R, Liu N, Long J, Qin F, Tang W, Yang Y, Chen Q, Yao S. Genome Biol. 2020 Aug 28;21(1):222. doi: 10.1186/s13059-020-02137-6. PubMed
Base editing: a brief review and a practical example. Choe DC, Musunuru K. J Biomed Res. 2020 Jul 31;35(2):107-114. doi: 10.7555/JBR.34.20200003. PubMed
Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo. Park H, Kim S. Nucleic Acids Res. 2021 Apr 6;49(6):e32. doi: 10.1093/nar/gkaa1231. PubMed Associated Plasmids pHyo094 (eMutaT7) pHyo182 pHyo245
Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes. Wang G, Li C, He S, Liu Z. Development. 2021 Mar 9;148(5). pii: dev.196899. doi: 10.1242/dev.196899. PubMed
Lineage tracing and analog recording in mammalian cells by single-site DNA writing. Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC. Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 10.1038/s41589-021-00769-8. Epub 2021 Mar 22. PubMed
The efficacy of CRISPR-mediated cytosine base editing with the RPS5a promoter in Arabidopsis thaliana. Choi M, Yun JY, Kim JH, Kim JS, Kim ST. Sci Rep. 2021 Apr 13;11(1):8087. doi: 10.1038/s41598-021-87669-y. PubMed
iPSC modeling of stage-specific leukemogenesis reveals BAALC as a key oncogene in severe congenital neutropenia. Dannenmann B, Klimiankou M, Oswald B, Solovyeva A, Mardan J, Nasri M, Ritter M, Zahabi A, Arreba-Tutusaus P, Mir P, Stein F, Kandabarau S, Lachmann N, Moritz T, Morishima T, Konantz M, Lengerke C, Ripperger T, Steinemann D, Erlacher M, Niemeyer CM, Zeidler C, Welte K, Skokowa J. Cell Stem Cell. 2021 May 6;28(5):906-922.e6. doi: 10.1016/j.stem.2021.03.023. Epub 2021 Apr 23. PubMed
A mutation in SLC37A4 causes a dominantly inherited congenital disorder of glycosylation characterized by liver dysfunction. Ng BG, Sosicka P, Fenaille F, Harroche A, Vuillaumier-Barrot S, Porterfield M, Xia ZJ, Wagner S, Bamshad MJ, Vergnes-Boiteux MC, Cholet S, Dalton S, Dell A, Dupre T, Fiore M, Haslam SM, Huguenin Y, Kumagai T, Kulik M, McGoogan K, Michot C, Nickerson DA, Pascreau T, Borgel D, Raymond K, Warad D, Flanagan-Steet H, Steet R, Tiemeyer M, Seta N, Bruneel A, Freeze HH. Am J Hum Genet. 2021 Jun 3;108(6):1040-1052. doi: 10.1016/j.ajhg.2021.04.013. Epub 2021 May 7. PubMed
Loss of DRC1 function leads to multiple morphological abnormalities of the sperm flagella and male infertility in human and mouse. Zhang J, He X, Wu H, Zhang X, Yang S, Liu C, Liu S, Hua R, Zhou S, Zhao S, Hu F, Zhang J, Liu W, Cheng H, Gao Y, Zhang F, Cao Y, Liu M. Hum Mol Genet. 2021 Oct 13;30(21):1996-2011. doi: 10.1093/hmg/ddab171. PubMed
Small-molecule compounds boost genome-editing efficiency of cytosine base editor. Zhao T, Li Q, Zhou C, Lv X, Liu H, Tu T, Tang N, Cheng Y, Liu X, Liu C, Zhao J, Song Z, Wang H, Li J, Gu F. Nucleic Acids Res. 2021 Sep 7;49(15):8974-8986. doi: 10.1093/nar/gkab645. PubMed
Optimization of C-to-G base editors with sequence context preference predictable by machine learning methods. Yuan T, Yan N, Fei T, Zheng J, Meng J, Li N, Liu J, Zhang H, Xie L, Ying W, Li D, Shi L, Sun Y, Li Y, Li Y, Sun Y, Zuo E. Nat Commun. 2021 Aug 12;12(1):4902. doi: 10.1038/s41467-021-25217-y. PubMed
Simultaneous high-efficiency base editing and reprogramming of patient fibroblasts. Jalil S, Keskinen T, Maldonado R, Sokka J, Trokovic R, Otonkoski T, Wartiovaara K. Stem Cell Reports. 2021 Nov 16. pii: S2213-6711(21)00552-X. doi: 10.1016/j.stemcr.2021.10.017. PubMed Associated Plasmids pRN3P_T3_ABEmax_IVT pRN3P_T3_BE4_IVT pRN3P_T3_BE3_IVT
Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE. Zhou J, Liu Y, Wei Y, Zheng S, Gou S, Chen T, Yang Y, Lan T, Chen M, Liao Y, Zhang Q, Tang C, Liu Y, Wu Y, Peng X, Gao M, Wang J, Zhang K, Lai L, Zou Q. Mol Ther. 2022 Jul 6;30(7):2443-2451. doi: 10.1016/j.ymthe.2022.04.010. Epub 2022 Apr 20. PubMed
PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency. Lau CH, Huang S, Lam RHW, Tin C. Mol Ther Methods Clin Dev. 2022 May 29;26:26-37. doi: 10.1016/j.omtm.2022.05.005. eCollection 2022 Sep 8. PubMed
Frequency and mechanisms of LINE-1 retrotransposon insertions at CRISPR/Cas9 sites. Tao J, Wang Q, Mendez-Dorantes C, Burns KH, Chiarle R. Nat Commun. 2022 Jun 27;13(1):3685. doi: 10.1038/s41467-022-31322-3. PubMed
Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotency. Luo L, Shi Y, Wang H, Wang Z, Dang Y, Li S, Wang S, Zhang K. PLoS Genet. 2022 Jul 5;18(7):e1010307. doi: 10.1371/journal.pgen.1010307. eCollection 2022 Jul. PubMed
Genome-wide base editor screen identifies regulators of protein abundance in yeast. Schubert OT, Bloom JS, Sadhu MJ, Kruglyak L. Elife. 2022 Nov 3;11. pii: 79525. doi: 10.7554/eLife.79525. PubMed Associated Plasmids pGal-BE3 pgRNA-backbone
Site-specific CRISPR-based mitochondrial DNA manipulation is limited by gRNA import. Schmiderer L, Yudovich D, Oburoglu L, Hjort M, Larsson J. Sci Rep. 2022 Nov 4;12(1):18687. doi: 10.1038/s41598-022-21794-0. PubMed
Mutation of S461, in the GOLGA3 phosphorylation site, does not affect mouse spermatogenesis. Xu C, Zhu M, Zhao S, Zhang X, Wang Y, Liu M. PeerJ. 2023 Apr 17;11:e15133. doi: 10.7717/peerj.15133. eCollection 2023. PubMed
Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation. Li Q, Lu J, Yin X, Chang Y, Wang C, Yan M, Feng L, Cheng Y, Gao Y, Xu B, Zhang Y, Wang Y, Cui G, Xu L, Sun Y, Zeng R, Li Y, Jing N, Xu GL, Wu L, Tang F, Li J. Nat Commun. 2023 May 22;14(1):2922. doi: 10.1038/s41467-023-38528-z. PubMed
PIE-seq: identifying RNA-binding protein targets by dual RNA-deaminase editing and sequencing. Ruan X, Hu K, Zhang X. Nat Commun. 2023 Jun 6;14(1):3275. doi: 10.1038/s41467-023-39054-8. PubMed Associated Plasmids pR008_APAD pR023_PIE-PUM2
APOBEC Reporter Systems for Evaluating diNucleotide Editing Levels. Rieffer AE, Chen Y, Salamango DJ, Moraes SN, Harris RS. CRISPR J. 2023 Oct;6(5):430-446. doi: 10.1089/crispr.2023.0027. Epub 2023 Sep 6. PubMed Associated Plasmids eGFP Y93H TC editing reporter eGFP Y93H AC editing reporter eGFP Y93H GC editing reporter eGFP Y93H CC editing reporter A3Bctd-L7G-Cas9n-UGI-NLS More ...
Single-base editing in IGF2 improves meat production and intramuscular fat deposition in Liang Guang Small Spotted pigs. Duo T, Liu X, Mo D, Bian Y, Cai S, Wang M, Li R, Zhu Q, Tong X, Liang Z, Jiang W, Chen S, Chen Y, He Z. J Anim Sci Biotechnol. 2023 Nov 2;14(1):141. doi: 10.1186/s40104-023-00930-4. PubMed
RNA splicing analysis deciphers developmental hierarchies and reveals therapeutic targets in adult glioma. Song X, Tiek D, Miki S, Huang T, Lu M, Goenka A, Iglesia R, Yu X, Wu R, Walker M, Zeng C, Shah H, Weng SHS, Huff A, Zhang W, Koga T, Hubert C, Horbinski CM, Furnari FB, Hu B, Cheng SY. J Clin Invest. 2024 Apr 25;134(11):e173789. doi: 10.1172/JCI173789. PubMed
Enhancing genome editing in hPSCs through dual inhibition of DNA damage response and repair pathways. Park JC, Kim YJ, Hwang GH, Kang CY, Bae S, Cha HJ. Nat Commun. 2024 May 11;15(1):4002. doi: 10.1038/s41467-024-48111-9. PubMed Associated Plasmids pCMV-AncBE4stem pCMV-PE4stem

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