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pETpRha-alpha-SkTm
(Plasmid #72929)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 72929 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET3a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4674
  • Total vector size (bp) 5529
  • Modifications to backbone
    T7 promoter replaced with Rhamnose inducible promoter
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha skeletal Tropomyosin
  • Alt name
    Tpm1.1
  • Species
    O. cuniculus (rabbit)
  • Insert Size (bp)
    855
  • GenBank ID
    NM_001105688.1 100125989
  • Promoter rhaB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer pBRrevBam_primer
  • 3′ sequencing primer T7 Terminator Primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETpRha-alpha-SkTm was a gift from Dan Mulvihill (Addgene plasmid # 72929 ; http://n2t.net/addgene:72929 ; RRID:Addgene_72929)

  • For your References section:

    An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput screen for molecules affecting alpha-synuclein oligomerisation. Eastwood T, Baker K, Brooker H, Frank S, Mulvihill DP. FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597. 10.1002/1873-3468.12597 PubMed 28214355
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