pNatA (pACYCduet-naa10-naa15)
(Plasmid #72928)

• Purpose: Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker
• Depositing Lab: Dan Mulvihill
• Publication: Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/1873-3468.12597.

Backbone

• Vector backbone: pACYCDUET
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 4008
• Total vector size (bp): 6376
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: naa10
• Species: S. pombe (fission yeast), Synthetic
• Insert Size (bp): 534
• Mutation: Codon optomised for E.coli expression
• Entrez Gene: naa10 (a.k.a. SPAC15E1.08, ard1)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde1 (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: DuetUP2 Primer
• 3′ sequencing primer: T7 Terminator Primer

Gene/Insert 2

• Gene/Insert name: naa15
• Species: S. pombe (fission yeast)
• Insert Size (bp): 2190
• Entrez Gene: naa15 (a.k.a. SPCC338.07c)
• Promoter: T7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sac1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: ACYCDuetUP1
• 3′ sequencing primer: DuetDOWN1 Primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry