pcDNA3-Cdt1 human
(Plasmid
#72681)
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Purposemammalian expression of human Cdt1
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72681 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3-HA C-terminal
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCdt1
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Alt namechromatin licensing and DNA replication factor 1
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SpeciesH. sapiens (human)
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Entrez GeneCDT1 (a.k.a. DUP, RIS2)
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGHrev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-Cdt1 human was a gift from Domenico Maiorano (Addgene plasmid # 72681 ; http://n2t.net/addgene:72681 ; RRID:Addgene_72681) -
For your References section:
PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, interfere with DNA polymerase eta and kappa focus formation on UV damage. Tsanov N, Kermi C, Coulombe P, Van der Laan S, Hodroj D, Maiorano D. Nucleic Acids Res. 2014 Apr;42(6):3692-706. doi: 10.1093/nar/gkt1400. Epub 2014 Jan 14. 10.1093/nar/gkt1400 PubMed 24423875