pKPY738
(Plasmid
#72674)
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PurposeC. elegans let-858 3' UTR (Gateway Entry Clone)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72674 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDONR P2R-P3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2639
- Total vector size (bp) 3049
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Vector typeGateway Entry Clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namelet-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
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SpeciesC. elegans (nematode)
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Insert Size (bp)410
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 Forward
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKPY738 was a gift from David Tirrell (Addgene plasmid # 72674 ; http://n2t.net/addgene:72674 ; RRID:Addgene_72674)
