pEGFP-centrin-1
(Plasmid #72641)

• Purpose: The plasmid encodes centrin-1 with an N-terminal EGFP tag
• Depositing Lab: Michel Bornens
• Publication: pEGFP-C1-centrin-1 (unpublished)

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4722
• Total vector size (bp): 5241
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: centrin-1
• Species: H. sapiens (human)
• Entrez Gene: CETN1 (a.k.a. CEN1, CETN)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: EGFP 3' Fw

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This plasmid was cloned by Yann ABRAHAM in the laboratory of Michel BORNENS
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was cloned by Yann ABRAHAM in the laboratory of Michel BORNENS