pmEos2-C1-centrin-1
(Plasmid #72588)

• Purpose: mEos2 photoconvertible fluorescent protein fused to the N-terminus of centrin-1
• Depositing Lab: Michel Bornens
• Publication: Sillibourne et al Cytoskeleton (Hoboken). 2011 Nov;68(11):619-27. doi: 10.1002/cm.20536. Epub 2011 Oct 18.

Backbone

• Vector backbone: pmEos2-C1
• Backbone manufacturer: modified vectors based on pEGFP-C1 from Clontech
• Backbone size w/o insert (bp): 4694
• Total vector size (bp): 5204
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: centrin 1
• Species: H. sapiens (human)
• Insert Size (bp): 519
• GenBank ID: NM_004066.1
• Entrez Gene: CETN1 (a.k.a. CEN1, CETN)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEos2 (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (not destroyed)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: mEos2 seq 2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated by removing the EGFP sequence from pEGFP-C1-centrin-1, originally generated by Yann ABRAHAM in the laboratory of Michel BORNENS, with mEos2