pJK544
(Plasmid
#72517)
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PurposeProduces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit (AaPurS)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 72517 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDFDuet-1
- Backbone size w/o insert (bp) 3781
- Total vector size (bp) 3954
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Streptomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameformylglycinamidine ribonucleotide synthetase, small subunit
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Alt namepurS
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Alt nameAZ09_06375
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SpeciesAcetobacter aceti 1023
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Mutationcontains additional Ala2
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GenBank IDKDE20458
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer ACYCDuetUP1
- 3′ sequencing primer DuetDOWN1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pCloDF13 replicon
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJK544 was a gift from T. J. Kappock (Addgene plasmid # 72517 ; http://n2t.net/addgene:72517 ; RRID:Addgene_72517)