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Addgene

pCDH-EF1-DIO-copGFP
(Plasmid #72253)

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Item Catalog # Description Quantity Price (USD)
Plasmid 72253 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pCDH-CMV-MCS
  • Backbone manufacturer
    Systembio
  • Total vector size (bp) 8164
  • Modifications to backbone
    ClaI to KpnI was removed and replaced with EF1 promoter and WPRE
  • Vector type
    Lentiviral, Cre/Lox

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    copGFP
  • Species
    Synthetic
  • Insert Size (bp)
    755
  • Promoter elongation factor-1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AscI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer GCA CTT GAT GTA ATT CTC C
  • 3′ sequencing primer CAACACCACGGAATTGTCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-DIO-copGFP was a gift from Kazuhiro Oka (Addgene plasmid # 72253 ; http://n2t.net/addgene:72253 ; RRID:Addgene_72253)
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