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VP12 Citations (26)

Originally described in: High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.
Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Keith Joung J Nature. 2016 Jan 6. doi: 10.1038/nature16526. PubMed Journal

Articles Citing VP12

Articles
A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research. Miano JM, Zhu QM, Lowenstein CJ. Arterioscler Thromb Vasc Biol. 2016 Jun;36(6):1058-75. doi: 10.1161/ATVBAHA.116.304790. Epub 2016 Apr 21. PubMed
Probing the impact of chromatin conformation on genome editing tools. Chen X, Rinsma M, Janssen JM, Liu J, Maggio I, Goncalves MA. Nucleic Acids Res. 2016 Jun 8. pii: gkw524. PubMed

Associated Plasmids
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage. Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E. Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8. PubMed

Associated Plasmids
Enhancer SINEs Link Pol III to Pol II Transcription in Neurons. Policarpi C, Crepaldi L, Brookes E, Nitarska J, French SM, Coatti A, Riccio A. Cell Rep. 2017 Dec 5;21(10):2879-2894. doi: 10.1016/j.celrep.2017.11.019. PubMed
A highly specific SpCas9 variant is identified by in vivo screening in yeast. Casini A, Olivieri M, Petris G, Montagna C, Reginato G, Maule G, Lorenzin F, Prandi D, Romanel A, Demichelis F, Inga A, Cereseto A. Nat Biotechnol. 2018 Jan 29. pii: nbt.4066. doi: 10.1038/nbt.4066. PubMed

Associated Plasmids
CRISPR/Cas9-Mediated Genome Editing for Huntington's Disease. Vachey G, Deglon N. Methods Mol Biol. 2018;1780:463-481. doi: 10.1007/978-1-4939-7825-0_21. PubMed
Optimized base editors enable efficient editing in cells, organoids and mice. Zafra MP, Schatoff EM, Katti A, Foronda M, Breinig M, Schweitzer AY, Simon A, Han T, Goswami S, Montgomery E, Thibado J, Kastenhuber ER, Sanchez-Rivera FJ, Shi J, Vakoc CR, Lowe SW, Tschaharganeh DF, Dow LE. Nat Biotechnol. 2018 Jul 3. pii: nbt.4194. doi: 10.1038/nbt.4194. PubMed

Associated Plasmids
Biallelic mutations in human NHLRC2 enhance myofibroblast differentiation in FINCA disease. Paakkola T, Salokas K, Miinalainen I, Lehtonen S, Manninen A, Kaakinen M, Ruddock LW, Varjosalo M, Kaarteenaho R, Uusimaa J, Hinttala R. Hum Mol Genet. 2018 Dec 15;27(24):4288-4302. doi: 10.1093/hmg/ddy298. PubMed
High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells. Idoko-Akoh A, Taylor L, Sang HM, McGrew MJ. Sci Rep. 2018 Oct 11;8(1):15126. doi: 10.1038/s41598-018-33244-x. PubMed

Associated Plasmids
Targeting of chondrocyte plasticity via connexin43 modulation attenuates cellular senescence and fosters a pro-regenerative environment in osteoarthritis. Varela-Eirin M, Varela-Vazquez A, Guitian-Caamano A, Paino CL, Mato V, Largo R, Aasen T, Tabernero A, Fonseca E, Kandouz M, Caeiro JR, Blanco A, Mayan MD. Cell Death Dis. 2018 Dec 5;9(12):1166. doi: 10.1038/s41419-018-1225-2. PubMed
Engineering Epigenetic Regulation Using Synthetic Read-Write Modules. Park M, Patel N, Keung AJ, Khalil AS. Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 10.1016/j.cell.2018.11.002. Epub 2018 Dec 6. PubMed

Associated Plasmids
Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNA(Gln)-processing system in human cells. He X, Wang Y, Yang F, Wang B, Xie H, Gu L, Zhao T, Liu X, Zhang D, Ren Q, Liu X, Liu Y, Gao C, Gu F. J Biol Chem. 2019 Jun 7;294(23):9308-9315. doi: 10.1074/jbc.RA119.007791. Epub 2019 Apr 22. PubMed
Genome Editing in Mammalian Cell Lines using CRISPR-Cas. Liu KI, Sutrisnoh NB, Wang Y, Tan MH. J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59086. PubMed
A Platform for Studying Neurodegeneration Mechanisms Using Genetically Encoded Biosensors. Ustyantseva EI, Medvedev SP, Vetchinova AS, Minina JM, Illarioshkin SN, Zakian SM. Biochemistry (Mosc). 2019 Mar;84(3):299-309. doi: 10.1134/S000629791903012X. PubMed
CTCF modulates allele-specific sub-TAD organization and imprinted gene activity at the mouse Dlk1-Dio3 and Igf2-H19 domains. Lleres D, Moindrot B, Pathak R, Piras V, Matelot M, Pignard B, Marchand A, Poncelet M, Perrin A, Tellier V, Feil R, Noordermeer D. Genome Biol. 2019 Dec 12;20(1):272. doi: 10.1186/s13059-019-1896-8. PubMed
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs. Kulcsar PI, Talas A, Toth E, Nyeste A, Ligeti Z, Welker Z, Welker E. Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5. PubMed

Associated Plasmids
Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs. Rose JC, Popp NA, Richardson CD, Stephany JJ, Mathieu J, Wei CT, Corn JE, Maly DJ, Fowler DM. Nat Commun. 2020 Jun 1;11(1):2697. doi: 10.1038/s41467-020-16542-9. PubMed
Germline engineering of the chicken genome using CRISPR/Cas9 by in vivo transfection of PGCs. Challagulla A, Jenkins KA, O'Neil TE, Morris KR, Wise TG, Tizard ML, Bean AGD, Schat KA, Doran TJ. Anim Biotechnol. 2020 Jul 24:1-10. doi: 10.1080/10495398.2020.1789869. PubMed
Cx43 and Associated Cell Signaling Pathways Regulate Tunneling Nanotubes in Breast Cancer Cells. Tishchenko A, Azorin DD, Vidal-Brime L, Munoz MJ, Arenas PJ, Pearce C, Girao H, Ramon Y Cajal S, Aasen T. Cancers (Basel). 2020 Sep 29;12(10). pii: cancers12102798. doi: 10.3390/cancers12102798. PubMed
In Vitro Validation of Transgene Expression in Gene-Edited Pigs Using CRISPR Transcriptional Activators. Polkoff KM, Chung J, Simpson SG, Gleason K, Piedrahita JA. CRISPR J. 2020 Oct;3(5):409-418. doi: 10.1089/crispr.2020.0037. PubMed
Modeling cell protrusion predicts how myosin II and actin turnover affect adhesion-based signaling. Chandra A, Butler MT, Bear JE, Haugh JM. Biophys J. 2022 Jan 4;121(1):102-118. doi: 10.1016/j.bpj.2021.11.2889. Epub 2021 Dec 1. PubMed
A rationalized definition of general tumor suppressor microRNAs excludes miR-34a. Mockly S, Houbron E, Seitz H. Nucleic Acids Res. 2022 Apr 26. pii: 6574673. doi: 10.1093/nar/gkac277. PubMed
LGR5 is a conserved marker of hair follicle stem cells in multiple species and is present early and throughout follicle morphogenesis. Polkoff KM, Gupta NK, Green AJ, Murphy Y, Chung J, Gleason KL, Simpson SG, Walker DM, Collins B, Piedrahita JA. Sci Rep. 2022 Jun 1;12(1):9104. doi: 10.1038/s41598-022-13056-w. PubMed
Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations. Langmuller AM, Champer J, Lapinska S, Xie L, Metzloff M, Champer SE, Liu J, Xu Y, Du J, Clark AG, Messer PW. Elife. 2022 Sep 22;11:e71809. doi: 10.7554/eLife.71809. PubMed
A LGR5 reporter pig model closely resembles human intestine for improved study of stem cells in disease. Schaaf CR, Polkoff KM, Carter A, Stewart AS, Sheahan B, Freund J, Ginzel J, Snyder JC, Roper J, Piedrahita JA, Gonzalez LM. FASEB J. 2023 Jun;37(6):e22975. doi: 10.1096/fj.202300223R. PubMed
A cleavage rule for selection of increased-fidelity SpCas9 variants with high efficiency and no detectable off-targets. Kulcsar PI, Talas A, Ligeti Z, Toth E, Rakvacs Z, Bartos Z, Krausz SL, Welker A, Vegi VL, Huszar K, Welker E. Nat Commun. 2023 Sep 16;14(1):5746. doi: 10.1038/s41467-023-41393-5. PubMed

Associated Plasmids

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