pBAD-TRP120
(Plasmid
#71732)
-
Purposeinducible expression of TRP120 in E. coli
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71732 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepBAD/Thio-TOPO
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4454
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTRP120
-
Alt namep120
-
SpeciesEhrlichia chaffeensis
-
Insert Size (bp)1644
-
GenBank IDWP_011452362
- Promoter pBAD
-
Tags
/ Fusion Proteins
- Thioredoxin (N terminal on backbone)
- 6xHIS (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer pBAD-F
- 3′ sequencing primer pTrcHis-R (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBAD-TRP120 was a gift from Jere McBride (Addgene plasmid # 71732 ; http://n2t.net/addgene:71732 ; RRID:Addgene_71732) -
For your References section:
Major species-specific antibody epitopes of the Ehrlichia chaffeensis p120 and E. canis p140 orthologs in surface-exposed tandem repeat regions. Luo T, Zhang X, McBride JW. Clin Vaccine Immunol. 2009 Jul;16(7):982-90. doi: 10.1128/CVI.00048-09. Epub 2009 May 6. 10.1128/CVI.00048-09 PubMed 19420187