VBS mutant of alpha catenin sensor
(Plasmid
#71710)
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PurposeThis form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71710 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDNA3.1
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Backbone manufacturerInvitrogen (Life Technologies)
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 9800
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namealpha-catenin ΔVBS FRET Sensor
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SpeciesH. sapiens (human)
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Insert Size (bp)4400
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MutationDeleted amino acid residues 316-405 in α -catenin and substituted with the homologous sequence from mouse vinculin (aa 514-606)
- Promoter CMV
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Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- YPET (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byacat 1-906 from David Rimm (Addgene plasmid # 24194)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid is a derived from the alpha catenin conformation sensor whose Vinculin binding site has been substituted with the homologous sequence from mouse vinculin (aa 514-606)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
VBS mutant of alpha catenin sensor was a gift from Deborah Leckband (Addgene plasmid # 71710 ; http://n2t.net/addgene:71710 ; RRID:Addgene_71710) -
For your References section:
Dynamic visualization of alpha-catenin reveals rapid, reversible conformation switching between tension states. Kim TJ, Zheng S, Sun J, Muhamed I, Wu J, Lei L, Kong X, Leckband DE, Wang Y. Curr Biol. 2015 Jan 19;25(2):218-24. doi: 10.1016/j.cub.2014.11.017. Epub 2014 Dec 24. 10.1016/j.cub.2014.11.017 PubMed 25544608