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Addgene

VBS mutant of alpha catenin sensor
(Plasmid #71710)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71710 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone manufacturer
    Invitrogen (Life Technologies)
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 9800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-catenin ΔVBS FRET Sensor
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4400
  • Mutation
    Deleted amino acid residues 316-405 in α -catenin and substituted with the homologous sequence from mouse vinculin (aa 514-606)
  • Promoter CMV
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • YPET (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    acat 1-906 from David Rimm (Addgene plasmid # 24194)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is a derived from the alpha catenin conformation sensor whose Vinculin binding site has been substituted with the homologous sequence from mouse vinculin (aa 514-606)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    VBS mutant of alpha catenin sensor was a gift from Deborah Leckband (Addgene plasmid # 71710 ; http://n2t.net/addgene:71710 ; RRID:Addgene_71710)
  • For your References section:

    Dynamic visualization of alpha-catenin reveals rapid, reversible conformation switching between tension states. Kim TJ, Zheng S, Sun J, Muhamed I, Wu J, Lei L, Kong X, Leckband DE, Wang Y. Curr Biol. 2015 Jan 19;25(2):218-24. doi: 10.1016/j.cub.2014.11.017. Epub 2014 Dec 24. 10.1016/j.cub.2014.11.017 PubMed 25544608