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Addgene

pORI28
(Plasmid #71595)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 71595 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pWV01
  • Backbone size (bp) 2200
  • Modifications to backbone
    RepA-
  • Vector type
    integration vector for bacteria

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    E. coli EC1000 provides RepA in trans
  • Growth instructions
    E. coli EC1000 required as it provides RepA in trans. This strain needs to be grown in 150 micrograms/mL erythromycin for pORI28 replication as well as 40 micrograms per mL of kanamycin to maintain repA in EC1000 chromosome). Please note that EC1000 (#71852) is available from Addgene and can be ordered at the same time for use as the cloning host of pORI28.
  • Copy number
    Low Copy

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was created by Jan Kok et al., Department of Genetics, Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 BB Haren, The Netherlands

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.

This plasmid has been found to be somewhat prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pORI28 was a gift from Todd Klaenhammer & Jan Kok (Addgene plasmid # 71595 ; http://n2t.net/addgene:71595 ; RRID:Addgene_71595)
  • For your References section:

    A general system for generating unlabelled gene replacements in bacterial chromosomes. Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J. Mol Gen Genet. 1996 Nov 27;253(1-2):217-24. PubMed 9003306