pORI28
(Plasmid #71595)

• Purpose: (Empty Backbone) EC1000 containing pORI28. Used as the source of plasmid pORI28. With pTRK669, part of a system for chromosomal integration in gram positive bacteria.
• Depositing Labs: Todd Klaenhammer, Jan Kok
• Publication: Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-24.

Backbone

• Vector backbone: pWV01
• Backbone size (bp): 2200
• Modifications to backbone: RepA-
• Vector type: integration vector for bacteria

Growth in Bacteria

• Bacterial Resistance(s): Erythromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): E. coli EC1000 provides RepA in trans
• Growth instructions: E. coli EC1000 required as it provides RepA in trans. This strain needs to be grown in 150 micrograms/mL erythromycin for pORI28 replication as well as 40 micrograms per mL of kanamycin to maintain repA in EC1000 chromosome). Please note that EC1000 (#71852) is available from Addgene and can be ordered at the same time for use as the cloning host of pORI28.
• Copy number: Low Copy

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• A portion of this plasmid was derived from a plasmid made by: This plasmid was created by Jan Kok et al., Department of Genetics, Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 BB Haren, The Netherlands

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.

This plasmid has been found to be somewhat prone to multimerization. Multimerization often does not impact plasmid function, but may reduce transformation efficiencies. You may need to screen multiple colonies to isolate the monomeric version of this plasmid. If you still have trouble isolating the monomeric version, you might consider linearizing, gel extracting, re-ligating, and transforming the plasmid.