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PurposeA positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 71471 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTT3
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Backbone manufacturerYves Durocher, NRC
- Backbone size w/o insert (bp) 6000
- Total vector size (bp) 8317
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCD200
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Alt nameOX-2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)714
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Entrez GeneCd200 (a.k.a. Cspmo2, MRCOX2, Mox2)
- Promoter CMV
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Tags
/ Fusion Proteins
- Cd4d3+4 (C terminal on insert)
- beta-lactamase (C terminal on insert)
- triple FLAG tag (C terminal on insert)
- 6xHis tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMW forward (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CD200-Cd4d3+4-COMP-blac-3xFLAG-6his was a gift from Gavin Wright (Addgene plasmid # 71471 ; http://n2t.net/addgene:71471 ; RRID:Addgene_71471) -
For your References section:
Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487