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Addgene

MSP7-bio
(Plasmid #47735)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47735 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher, NRC
  • Backbone size w/o insert (bp) 6633
  • Total vector size (bp) 7605
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Codon-optimised MSP7
  • Species
    Synthetic; Plasmodium falciparum
  • Insert Size (bp)
    972
  • Mutation
    Exogenous signal peptide of mouse origin, changed Serine131 and Serine278 to Alanine residues
  • GenBank ID
  • Promoter CMV
  • Tags / Fusion Proteins
    • rat Cd4 d3+4 (C terminal on backbone)
    • enzymatic biotinylation sequence (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer CMV-fwd
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained under an MTA from Yves Durocher, National Research Council of Canada- Biotechnology Research Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MSP7-bio was a gift from Gavin Wright (Addgene plasmid # 47735 ; http://n2t.net/addgene:47735 ; RRID:Addgene_47735)
  • For your References section:

    A library of functional recombinant cell surface and secreted Plasmodium falciparum merozoite proteins. Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, Wright GJ. Mol Cell Proteomics. 2013 Sep 16. 10.1074/mcp.O113.028357 PubMed 24043421