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Purpose(Empty Backbone) Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIL253
- Backbone size (bp) 4963
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Modifications to backboneinsertion pSA34, pIC-19R MCS
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Vector typeBacterial Expression ; E. coli-gram positive bacteria shuttle vector
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM110
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Growth instructionsErythromycin selection is 150 ug/ml in E. coli
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Addgene Notes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTRKH2 was a gift from Rodolphe Barrangou & Todd Klaenhammer (Addgene plasmid # 71312 ; http://n2t.net/addgene:71312 ; RRID:Addgene_71312) -
For your References section:
High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening. O'Sullivan DJ, Klaenhammer TR. Gene. 1993 Dec 31;137(2):227-31. PubMed 8299952
Map uploaded by the depositor.
