pRL2960
(Plasmid
#70694)
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Purpose(Empty Backbone) pDU1-E. coli shuttle vector (Km-r) with extended polylinker
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70694 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRL25
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Backbone manufacturerWolk Lab
- Backbone size (bp) 7432
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Vector typeBacterial shuttle vector
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Selectable markersNeomycin (select with G418) ; for cyanobacteria
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
MCS: BamHI-AscI-SrfI-XhoI(PspXI)-BsiWI-AatII(ZraI)-AvrII-StuI-NsiI-SacII-ApaI-ClaI-EcoRI-SacI(EcoICRI)-KpnI(Acc65I)-SalI-SbfI-NotI
Medium copy number in E. coli. The literature (e.g., Yang et al., Res. in Microbiol. [Inst. Pasteur] 164, 127-135, 2013) says that there is substantial variability in the copy number of pDU1-based plasmids in Anabaena PCC 7120 dependent on the insert in the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRL2960 was a gift from Peter Wolk (Addgene plasmid # 70694 ; http://n2t.net/addgene:70694 ; RRID:Addgene_70694) -
For your References section:
Paired cloning vectors for complementation of mutations in the cyanobacterium Anabaena sp. strain PCC 7120. Wolk CP, Fan Q, Zhou R, Huang G, Lechno-Yossef S, Kuritz T, Wojciuch E. Arch Microbiol. 2007 Dec;188(6):551-63. Epub 2007 Jul 17. 10.1007/s00203-007-0276-z PubMed 17639350
Map uploaded by the depositor.
