pRL277
(Plasmid #70255)

• Purpose: (Empty Backbone) sacB plasmid with polylinker; streptomycin/spectinomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.
• Depositing Lab: Peter Wolk
• Publication: Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

Backbone

• Vector backbone: see comments
• Backbone size (bp): 6798
• Vector type: Conditionally lethal
• Selectable markers: spectinomycin for cyanobacteria

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: We consider it wise to store plasmids bearing a sacB gene at -80 deg. and to assess the very suspension that will be stored to be sure that it is highly sensitive to sucrose, and, similarly, to test individual colonies from which a sacB-bearing plasmid will be derived to be certain that sacB has retained great sensitivity to sucrose.
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: see comments

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

sacB was derived from pUM24 (Ried, J. L., and A. Colimer Gene 57:239-246, 1987) and from pUCD800 (Gay, et al. 1985 J. Bacteriol. 164:918-921).
The streptomycin/spectinomycin determinant in pRL277 was derived from the omega cassette (Prentki, et al. Gene 103: 17-23 1991).

Additional Reference for this plasmid:
Black TA, Cai Y, Wolk CP (1993) Spatial expression and autoregulation of hetR, a gene involved in the control of heterocyst development in Anabaena. Mol Microbiol 9: 77-84. Publisher’s correction (1993): Mol Microbiol 10: 1153