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Purpose(Empty Backbone) Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 70186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRetroX-Tight-Pur
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Backbone manufacturerClontech
- Backbone size (bp) 7141
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Modifications to backboneReplaced Puromycin resistance gene with OgNLuc LumiFluor.
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Vector typeMammalian Expression, Retroviral
- Promoter PGK
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CTGGGCTCAGAGGCTGGG
- 3′ sequencing primer CCTACAGGTGGGGTCTTTC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRetroX-Tight-MCS_PGK-OgNLuc was a gift from Antonio Amelio (Addgene plasmid # 70186 ; http://n2t.net/addgene:70186 ; RRID:Addgene_70186) -
For your References section:
Fluorophore-NanoLuc BRET Reporters Enable Sensitive In Vivo Optical Imaging and Flow Cytometry for Monitoring Tumorigenesis. Schaub FX, Reza MS, Flaveny CA, Li W, Musicant AM, Hoxha S, Guo M, Cleveland JL, Amelio AL. Cancer Res. 2015 Sep 30. pii: canres.3538.2014. 10.1158/0008-5472.CAN-14-3538 PubMed 26424696
Map uploaded by the depositor.
