pRetroX-Tight-MCS_PGK-OgNLuc
(Plasmid #70186)

• Purpose: (Empty Backbone) Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.
• Depositing Lab: Antonio Amelio
• Publication: Schaub et al Cancer Res. 2015 Sep 30. pii: canres.3538.2014.

Backbone

• Vector backbone: pRetroX-Tight-Pur
• Backbone manufacturer: Clontech
• Backbone size (bp): 7141
• Modifications to backbone: Replaced Puromycin resistance gene with OgNLuc LumiFluor.
• Vector type: Mammalian Expression, Retroviral
• Promoter: PGK

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTGGGCTCAGAGGCTGGG
• 3′ sequencing primer: CCTACAGGTGGGGTCTTTC

Resource Information

• Supplemental Documents:
  • Annotated GenBank file
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • NanoLuc Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry