pZ001 (AacC2c1 locus)
(Plasmid
#70166)
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PurposeExpresses AacC2c1 full locus
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70166 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC184
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Backbone manufacturerNEB
- Backbone size w/o insert (bp) 4245
- Total vector size (bp) 10737
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAacC2c1 locus
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Alt nameAacCas12b
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Speciesalicyclobacillus acidoterrestris
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Insert Size (bp)6680
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GenBank IDWP_021296342.1
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZ001 (AacC2c1 locus) was a gift from Feng Zhang (Addgene plasmid # 70166 ; http://n2t.net/addgene:70166 ; RRID:Addgene_70166) -
For your References section:
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems. Shmakov S, Abudayyeh OO, Makarova KS, Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV. Mol Cell. 2015 Nov 5;60(3):385-97. doi: 10.1016/j.molcel.2015.10.008. Epub 2015 Oct 22. 10.1016/j.molcel.2015.10.008 PubMed 26593719