p5E-mfap4
(Plasmid #70052)

• Purpose: 5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.
• Depositing Lab: David Tobin
• Publication: Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015.

Backbone

• Vector backbone: p5E-MCS
• Backbone size w/o insert (bp): 2765
• Total vector size (bp): 4367
• Vector type: Gateway 5' Element

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mfap4 promoter
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1602

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13F (-21)
• 3′ sequencing primer: M13R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Addgene's quality control sequencing has identified a number of single-nucleotide discrepancies, compared to the NCBI Genbank reference sequence for the Zebrafish mfap4 promoter. The depositing laboratory is aware of these differences, and states that they are polymorphisms. This plasmid accurately reflects the construct as it was used in the associated publication.