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Purpose(Empty Backbone) High yield protein secretion in Aspergillus nidulans
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70030 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepUC19
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Backbone manufacturerNew England Bioscienes
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Vector typeSynthetic Biology
- Promoter A. niger glucoamylase promoter (glaAp)4
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Note: Compared to the depositing laboratory's provided full plasmid sequence, Addgene's quality control sequencing finds a few single nucleotide differences.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEXPYR was a gift from Rolf Prade (Addgene plasmid # 70030 ; http://n2t.net/addgene:70030 ; RRID:Addgene_70030) -
For your References section:
High-yield secretion of multiple client proteins in Aspergillus. Segato F, Damasio AR, Goncalves TA, de Lucas RC, Squina FM, Decker SR, Prade RA. Enzyme Microb Technol. 2012 Jul 15;51(2):100-6. doi: 10.1016/j.enzmictec.2012.04.008. Epub 2012 May 2. 10.1016/j.enzmictec.2012.04.008 PubMed 22664194