pRR-S4
(Plasmid
#70000)
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PurposeMammalian expression of cytochrome p450 CYP2B6 variant
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 70000 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCYP2B6
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Alt name2B6.279P
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Alt namecytochrome P450, family 2, subfamily B, polypeptide 6
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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MutationG835C (A279P)
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Entrez GeneCYP2B6 (a.k.a. CPB6, CYP2B, CYP2B7, CYP2B7P, CYPIIB6, EFVM, IIB1, P450)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRR-S4 was a gift from Kathrin Klein (Addgene plasmid # 70000 ; http://n2t.net/addgene:70000 ; RRID:Addgene_70000) -
For your References section:
Novel CYP2B6 enzyme variants in a Rwandese population: functional characterization and assessment of in silico prediction tools. Radloff R, Gras A, Zanger UM, Masquelier C, Arumugam K, Karasi JC, Arendt V, Seguin-Devaux C, Klein K. Hum Mutat. 2013 May;34(5):725-34. doi: 10.1002/humu.22295. Epub 2013 Mar 11. 10.1002/humu.22295 PubMed 23418033