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pY016 (pcDNA3.1-hLbCpf1) Citations (40)

Originally described in: Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System.
Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. PubMed Journal

Articles Citing pY016 (pcDNA3.1-hLbCpf1)

Articles
Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human cells. Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, McCaw ZR, Aryee MJ, Joung JK. Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620. PubMed

Associated Plasmids
Cpf1 nucleases demonstrate robust activity to induce DNA modification by exploiting homology directed repair pathways in mammalian cells. Toth E, Weinhardt N, Bencsura P, Huszar K, Kulcsar PI, Talas A, Fodor E, Welker E. Biol Direct. 2016 Sep 14;11:46. doi: 10.1186/s13062-016-0147-0. PubMed

Associated Plasmids
In vivo high-throughput profiling of CRISPR-Cpf1 activity. Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, Koh HC, Nam JW, Kim H. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. PubMed

Associated Plasmids
CRISPR-Cpf1 correction of muscular dystrophy mutations in human cardiomyocytes and mice. Zhang Y, Long C, Li H, McAnally JR, Baskin KK, Shelton JM, Bassel-Duby R, Olson EN. Sci Adv. 2017 Apr 12;3(4):e1602814. doi: 10.1126/sciadv.1602814. eCollection 2017 Apr. PubMed
Cpf1 proteins excise CRISPR RNAs from mRNA transcripts in mammalian cells. Zhong G, Wang H, Li Y, Tran MH, Farzan M. Nat Chem Biol. 2017 Aug;13(8):839-841. doi: 10.1038/nchembio.2410. Epub 2017 Jun 19. PubMed
Targeted Disruption of V600E-Mutant BRAF Gene by CRISPR-Cpf1. Yang M, Wei H, Wang Y, Deng J, Tang Y, Zhou L, Guo G, Tong A. Mol Ther Nucleic Acids. 2017 Sep 15;8:450-458. doi: 10.1016/j.omtn.2017.05.009. Epub 2017 May 17. PubMed
Enhanced Genome Editing Tools For Multi-Gene Deletion Knock-Out Approaches Using Paired CRISPR sgRNAs in CHO Cells. Schmieder V, Bydlinski N, Strasser R, Baumann M, Kildegaard HF, Jadhav V, Borth N. Biotechnol J. 2018 Mar;13(3):e1700211. doi: 10.1002/biot.201700211. Epub 2017 Nov 13. PubMed
A 'new lease of life': FnCpf1 possesses DNA cleavage activity for genome editing in human cells. Tu M, Lin L, Cheng Y, He X, Sun H, Xie H, Fu J, Liu C, Li J, Chen D, Xi H, Xue D, Liu Q, Zhao J, Gao C, Song Z, Qu J, Gu F. Nucleic Acids Res. 2017 Nov 2;45(19):11295-11304. doi: 10.1093/nar/gkx783. PubMed
Drug-tunable multidimensional synthetic gene control using inducible degron-tagged dCas9 effectors. Kleinjan DA, Wardrope C, Nga Sou S, Rosser SJ. Nat Commun. 2017 Oct 30;8(1):1191. doi: 10.1038/s41467-017-01222-y. PubMed
Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1. Kweon J, Jang AH, Kim DE, Yang JW, Yoon M, Rim Shin H, Kim JS, Kim Y. Nat Commun. 2017 Nov 23;8(1):1723. doi: 10.1038/s41467-017-01650-w. PubMed
Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA. Ferenczi A, Pyott DE, Xipnitou A, Molnar A. Proc Natl Acad Sci U S A. 2017 Dec 19;114(51):13567-13572. doi: 10.1073/pnas.1710597114. Epub 2017 Dec 5. PubMed
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. Nat Commun. 2017 Dec 8;8(1):2024. doi: 10.1038/s41467-017-01836-2. PubMed

Associated Plasmids
Engineering Introns to Express RNA Guides for Cas9- and Cpf1-Mediated Multiplex Genome Editing. Ding D, Chen K, Chen Y, Li H, Xie K. Mol Plant. 2018 Apr 2;11(4):542-552. doi: 10.1016/j.molp.2018.02.005. Epub 2018 Feb 17. PubMed

Associated Plasmids
Small molecules promote CRISPR-Cpf1-mediated genome editing in human pluripotent stem cells. Ma X, Chen X, Jin Y, Ge W, Wang W, Kong L, Ji J, Guo X, Huang J, Feng XH, Fu J, Zhu S. Nat Commun. 2018 Apr 3;9(1):1303. doi: 10.1038/s41467-018-03760-5. PubMed
Design and assessment of engineered CRISPR-Cpf1 and its use for genome editing. Li B, Zeng C, Dong Y. Nat Protoc. 2018 May;13(5):899-914. doi: 10.1038/nprot.2018.004. Epub 2018 Apr 5. PubMed
Generation of the Adenovirus Vector-Mediated CRISPR/Cpf1 System and the Application for Primary Human Hepatocytes Prepared from Humanized Mice with Chimeric Liver. Tsukamoto T, Sakai E, Iizuka S, Taracena-Gandara M, Sakurai F, Mizuguchi H. Biol Pharm Bull. 2018;41(7):1089-1095. doi: 10.1248/bpb.b18-00222. PubMed
Discovery of widespread Type I and Type V CRISPR-Cas inhibitors. Marino ND, Zhang JY, Borges AL, Sousa AA, Leon LM, Rauch BJ, Walton RT, Berry JD, Joung JK, Kleinstiver BP, Bondy-Denomy J. Science. 2018 Sep 6. pii: science.aau5174. doi: 10.1126/science.aau5174. PubMed

Associated Plasmids
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3'-overhang. Bin Moon S, Lee JM, Kang JG, Lee NE, Ha DI, Kim DY, Kim SH, Yoo K, Kim D, Ko JH, Kim YS. Nat Commun. 2018 Sep 7;9(1):3651. doi: 10.1038/s41467-018-06129-w. PubMed
Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA. Gao Z, Herrera-Carrillo E, Berkhout B. RNA Biol. 2018;15(12):1458-1467. doi: 10.1080/15476286.2018.1551703. Epub 2018 Nov 29. PubMed
Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing. Kleinstiver BP, Sousa AA, Walton RT, Tak YE, Hsu JY, Clement K, Welch MM, Horng JE, Malagon-Lopez J, Scarfo I, Maus MV, Pinello L, Aryee MJ, Joung JK. Nat Biotechnol. 2019 Feb 11. pii: 10.1038/s41587-018-0011-0. doi: 10.1038/s41587-018-0011-0. PubMed

Associated Plasmids
Knockout rat models mimicking human atherosclerosis created by Cpf1-mediated gene targeting. Lee JG, Ha CH, Yoon B, Cheong SA, Kim G, Lee DJ, Woo DC, Kim YH, Nam SY, Lee SW, Sung YH, Baek IJ. Sci Rep. 2019 Feb 22;9(1):2628. doi: 10.1038/s41598-019-38732-2. PubMed
Enhanced Cas12a editing in mammalian cells and zebrafish. Liu P, Luk K, Shin M, Idrizi F, Kwok S, Roscoe B, Mintzer E, Suresh S, Morrison K, Frazao JB, Bolukbasi MF, Ponnienselvan K, Luban J, Zhu LJ, Lawson ND, Wolfe SA. Nucleic Acids Res. 2019 Mar 20. pii: 5403491. doi: 10.1093/nar/gkz184. PubMed

Associated Plasmids
Assessment of Cas12a-mediated gene editing efficiency in plants. Bernabe-Orts JM, Casas-Rodrigo I, Minguet EG, Landolfi V, Garcia-Carpintero V, Gianoglio S, Vazquez-Vilar M, Granell A, Orzaez D. Plant Biotechnol J. 2019 Apr 5. doi: 10.1111/pbi.13113. PubMed

Associated Plasmids
EXP1 is required for organisation of EXP2 in the intraerythrocytic malaria parasite vacuole. Nessel T, Beck JM, Rayatpisheh S, Jami-Alahmadi Y, Wohlschlegel JA, Goldberg DE, Beck JR. Cell Microbiol. 2020 Jan 28:e13168. doi: 10.1111/cmi.13168. PubMed

Associated Plasmids
A fly model establishes distinct mechanisms for synthetic CRISPR/Cas9 sex distorters. Fasulo B, Meccariello A, Morgan M, Borufka C, Papathanos PA, Windbichler N. PLoS Genet. 2020 Mar 13;16(3):e1008647. doi: 10.1371/journal.pgen.1008647. eCollection 2020 Mar. PubMed
A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing. Chen P, Zhou J, Wan Y, Liu H, Li Y, Liu Z, Wang H, Lei J, Zhao K, Zhang Y, Wang Y, Zhang X, Yin L. Genome Biol. 2020 Mar 25;21(1):78. doi: 10.1186/s13059-020-01989-2. PubMed
CRISPR-Cas12a delivery by DNA-mediated bioresponsive editing for cholesterol regulation. Sun W, Wang J, Hu Q, Zhou X, Khademhosseini A, Gu Z. Sci Adv. 2020 May 20;6(21):eaba2983. doi: 10.1126/sciadv.aba2983. eCollection 2020 May. PubMed
Design and Evaluation of Guide RNA Transcripts with a 3'-Terminal HDV Ribozyme to Enhance CRISPR-Based Gene Inactivation. Berkhout B, Gao Z, Herrera-Carrillo E. Methods Mol Biol. 2021;2167:205-224. doi: 10.1007/978-1-0716-0716-9_12. PubMed
CRISPR/Cas12a Mediated Genome Editing Enhances Bombyx mori Resistance to BmNPV. Dong Z, Qin Q, Hu Z, Zhang X, Miao J, Huang L, Chen P, Lu C, Pan M. Front Bioeng Biotechnol. 2020 Jul 15;8:841. doi: 10.3389/fbioe.2020.00841. eCollection 2020. PubMed
Gene Manipulation Using Fusion Guide RNAs for Cas9 and Cas12a. Shin HR, Kweon J, Kim Y. Methods Mol Biol. 2021;2162:185-193. doi: 10.1007/978-1-0716-0687-2_10. PubMed
A more efficient CRISPR-Cas12a variant derived from Lachnospiraceae bacterium MA2020. Tran MH, Park H, Nobles CL, Karunadharma P, Pan L, Zhong G, Wang H, He W, Ou T, Crynen G, Sheptack K, Stiskin I, Mou H, Farzan M. Mol Ther Nucleic Acids. 2021 Feb 18;24:40-53. doi: 10.1016/j.omtn.2021.02.012. eCollection 2021 Jun 4. PubMed
Engineered sex ratio distortion by X-shredding in the global agricultural pest Ceratitis capitata. Meccariello A, Krsticevic F, Colonna R, Del Corsano G, Fasulo B, Papathanos PA, Windbichler N. BMC Biol. 2021 Apr 16;19(1):78. doi: 10.1186/s12915-021-01010-7. PubMed
Cas12a variants designed for lower genome-wide off-target effect through stringent PAM recognition. Zhou J, Chen P, Wang H, Liu H, Li Y, Zhang Y, Wu Y, Paek C, Sun Z, Lei J, Yin L. Mol Ther. 2022 Jan 5;30(1):244-255. doi: 10.1016/j.ymthe.2021.10.010. Epub 2021 Oct 20. PubMed
Reprogramming of the heavy-chain CDR3 regions of a human antibody repertoire. Ou T, He W, Quinlan BD, Guo Y, Tran MH, Karunadharma P, Park H, Davis-Gardner ME, Yin Y, Zhang X, Wang H, Zhong G, Farzan M. Mol Ther. 2022 Jan 5;30(1):184-197. doi: 10.1016/j.ymthe.2021.10.027. Epub 2021 Nov 2. PubMed
The heat is on: a simple method to increase genome editing efficiency in plants. Blomme J, Develtere W, Kose A, Arraiza Ribera J, Brugmans C, Jaraba-Wallace J, Decaestecker W, Rombaut D, Baekelandt A, Daniel Fernandez Fernandez A, Van Breusegem F, Inze D, Jacobs T. BMC Plant Biol. 2022 Mar 24;22(1):142. doi: 10.1186/s12870-022-03519-7. PubMed
Combination of Isothermal Recombinase-Aided Amplification and CRISPR-Cas12a-Mediated Assay for Rapid Detection of Major Severe Acute Respiratory Syndrome Coronavirus 2 Variants of Concern. Lin H, Liang Y, Zou L, Li B, Zhao J, Wang H, Sun J, Deng X, Tang S. Front Microbiol. 2022 Jun 28;13:945133. doi: 10.3389/fmicb.2022.945133. eCollection 2022. PubMed
gDesigner: computational design of synthetic gRNAs for Cas12a-based transcriptional repression in mammalian cells. Crone MA, MacDonald JT, Freemont PS, Siciliano V. NPJ Syst Biol Appl. 2022 Sep 16;8(1):34. doi: 10.1038/s41540-022-00241-w. PubMed
RNA export through the nuclear pore complex is directional. Ashkenazy-Titelman A, Atrash MK, Boocholez A, Kinor N, Shav-Tal Y. Nat Commun. 2022 Oct 6;13(1):5881. doi: 10.1038/s41467-022-33572-7. PubMed
Characterization of a thermostable Cas12a ortholog. Wu J, Gao P, Shi Y, Zhang C, Tong X, Fan H, Zhou X, Zhang Y, Yin H. Cell Insight. 2023 Oct 11;2(6):100126. doi: 10.1016/j.cellin.2023.100126. eCollection 2023 Dec. PubMed
Harnessing noncanonical crRNAs to improve functionality of Cas12a orthologs. Nguyen LT, Macaluso NC, Rakestraw NR, Carman DR, Pizzano BLM, Hautamaki RC, Rananaware SR, Roberts IE, Jain PK. Cell Rep. 2024 Feb 27;43(2):113777. doi: 10.1016/j.celrep.2024.113777. Epub 2024 Feb 14. PubMed

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