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Addgene

pEGFP-N1 VAMP2 R125TAG
(Plasmid #69876)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69876 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Total vector size (bp) 5068
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAMP2-GFP
  • Alt name
    synaptobrevin 2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1119
  • Mutation
    Amber stop codon in the linker between VAMP2 and GFP (R125TAG)
  • Entrez Gene
    Vamp2 (a.k.a. RATVAMPB, RATVAMPIR, SYB, Syb2)
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1 VAMP2 R125TAG was a gift from Silvio Rizzoli (Addgene plasmid # 69876 ; http://n2t.net/addgene:69876 ; RRID:Addgene_69876)

  • For your References section:

    Super-resolution Microscopy of Clickable Amino Acids Reveals the Effects of Fluorescent Protein Tagging on Protein Assemblies. Vreja IC, Nikic I, Gottfert F, Bates M, Krohnert K, Outeiro TF, Hell SW, Lemke EA, Rizzoli SO. ACS Nano. 2015 Nov 24;9(11):11034-41. doi: 10.1021/acsnano.5b04434. Epub 2015 Oct 28. 10.1021/acsnano.5b04434 PubMed 26498474
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