pCAP03-acc(3)IV
(Plasmid #69862)

• Purpose: (Empty Backbone) pCAP03-acc(3)IV is a upgraded version of the pCAP01(Plasmid #59981)
• Depositing Lab: Bradley Moore
• Publication: Tang et al ACS Chem Biol. 2015 Oct 21.

Backbone

• Vector backbone: pCAP01
• Modifications to backbone: URA3 gene was introduced into pCAP01 under the strong promoter of the Schizosaccharomyces pombe ADH1 gene (pADH1) into the SpeI and KpnI restriction sites of pCAP01, as a counter selectable marker, to produce pCAP03. pADH1 can tolerate an insertion of up to 130 bp between the TATA box and the transcription initiation site. This method allows the use of shorter capture arms and conveniently selects against non-homologous end joining in the presence of 5-fluoroorotic acid (5-FOA). In order to easy linearize pCAP03, the 1369 bp apramycin resistance cassette (acc(3)IV) was amplified from pIJ773 and cloned into the pCAP03 to obtain pCAP03-acc(3)IV .
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: TRP1, URA3 ; apramycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The vector also contain the apramycin resistance gene from pIJ773
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • Direct Cloning and Heterologous Expression of Biosynthetic Gene Cluster
• A portion of this plasmid was derived from a plasmid made by: Ura3 and the strong promoter of the Schizosaccharomyces pombe ADH1 gene (pADH1) were cloned from pARS-VN provided by Dr. Larionov V. (National Cancer Institute, NIH).
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Noskov, V. N., Kouprina, N., Leem, S. H., Ouspenski, I., Barrett, J. C., and Larionov, V. (2003) A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast, BMC Genomics 4, 16.