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Addgene

HPRT2b-pGEM
(Plasmid #69539)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69539 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HPRT gRNA2
  • gRNA/shRNA sequence
    5'-GACTGTAAGTGAATTACTT-3'
  • Species
    H. sapiens (human)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HPRT2b-pGEM was a gift from Hong Yan (Addgene plasmid # 69539 ; http://n2t.net/addgene:69539 ; RRID:Addgene_69539)

  • For your References section:

    Enriching CRISPR-Cas9 targeted cells by co-targeting the HPRT gene. Liao S, Tammaro M, Yan H. Nucleic Acids Res. 2015 Jun 29. pii: gkv675. 10.1093/nar/gkv675 PubMed 26130722
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