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L-CRISPR-CTN (MLL-ctrl)
(Plasmid #69215)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69215 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    SIN40C
  • Modifications to backbone
    modified pLKO5
  • Vector type
    Lentiviral, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SpCas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4300
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Sp sgRNA scaffold
  • Insert Size (bp)
    75

Gene/Insert 3

  • Gene/Insert name
    SFFV promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    250

Gene/Insert 4

  • Gene/Insert name
    P2A-mNeonGreen
  • Species
    Synthetic
  • Insert Size (bp)
    750

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L-CRISPR-CTN (MLL-ctrl) was a gift from Dirk Heckl (Addgene plasmid # 69215 ; http://n2t.net/addgene:69215 ; RRID:Addgene_69215)

  • For your References section:

    CRISPR-Cas9-induced t(11;19)/MLL-ENL translocations initiate leukemia in human hematopoietic progenitor cells in vivo. Reimer J, Knoess S, Labuhn M, Charpentier EM, Gohring G, Schlegelberger B, Klusmann JH, Heckl D. Haematologica. 2017 Jun 1. pii: haematol.2017.164046. doi: 10.3324/haematol.2017.164046. 10.3324/haematol.2017.164046 PubMed 28572162
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