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Purpose(Empty Backbone) regulated expression of genes in Escherichia coli
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 69122 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonebased on pUC18
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Modifications to backboneadded tac promoter, rrnB transcription terminator, polylinker and lacZalpha fragment from pUC18, and lacIQ gene.
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Vector typeBacterial Expression
- Promoter tac
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13pUC-Rev
- 3′ sequencing primer M13pUC-Fwd (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The depositor sequence is the full sequence for this plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pttq18 was a gift from Michael Stark (Addgene plasmid # 69122 ; http://n2t.net/addgene:69122 ; RRID:Addgene_69122) -
For your References section:
Multicopy expression vectors carrying the lac repressor gene for regulated high-level expression of genes in Escherichia coli. Stark MJ. Gene. 1987;51(2-3):255-67. PubMed 3110013
Map uploaded by Addgene staff.
