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Addgene

pLPC-S22A-Progerin
(Plasmid #69065)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 69065 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLPC
  • Backbone size w/o insert (bp) 5493
  • Total vector size (bp) 7338
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Progerin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1845
  • Mutation
    Progerin with serine 22 mutated to alanine
  • Entrez Gene
    LMNA (a.k.a. CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1)
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer taggcgtgtacggtggga
  • 3′ sequencing primer acctgtaggtttggcaag
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLPC-S22A-Progerin was a gift from Gerardo Ferbeyre (Addgene plasmid # 69065 ; http://n2t.net/addgene:69065 ; RRID:Addgene_69065)

  • For your References section:

    Mutant lamin A links prophase to a p53 independent senescence program. Moiseeva O, Lessard F, Acevedo-Aquino M, Vernier M, Tsantrizos YS, Ferbeyre G. Cell Cycle. 2015 Aug 3;14(15):2408-21. doi: 10.1080/15384101.2015.1053671. Epub 2015 Jun 1. 10.1080/15384101.2015.1053671 PubMed 26029982
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