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Addgene

bactin2:loxP-STOP-loxP-DsRed-express
(Plasmid #68797)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68797 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pExpress1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    beta-actin promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    11000

Gene/Insert 2

  • Gene/Insert name
    loxp-stop-codon-loxp-dsred

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found that plasmid contains 3x SV40pA repeats rather than the expected 4x as described in associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    bactin2:loxP-STOP-loxP-DsRed-express was a gift from David Traver (Addgene plasmid # 68797 ; http://n2t.net/addgene:68797 ; RRID:Addgene_68797)
  • For your References section:

    Haematopoietic stem cells derive directly from aortic endothelium during development. Bertrand JY, Chi NC, Santoso B, Teng S, Stainier DY, Traver D. Nature. 2010 Mar 4;464(7285):108-11. doi: 10.1038/nature08738. Epub 2010 Feb 14. 10.1038/nature08738 PubMed 20154733