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PurposeMultisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 68339 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDestattB
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Backbone manufacturerMosimann lab, Addgene plasmid #68313
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Vector typeZebrafish transgenesis
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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SpeciesAequorea victoria
- Promoter ubi
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer Lac promoter (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDestattB_ubi:EGFP (pCM272) was a gift from Christian Mosimann & Leonard Zon (Addgene plasmid # 68339 ; http://n2t.net/addgene:68339 ; RRID:Addgene_68339) -
For your References section:
Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system. Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI. Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 Jul 3. 10.1002/dvdy.23989 PubMed 23723152