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Addgene

pENTR5'-attP (pCM254)
(Plasmid #68311)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68311 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pENTR/D
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2820
  • Vector type
    Gateway entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    attP
  • Species
    Synthetic
  • Insert Size (bp)
    220

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR5'-attP (pCM254) was a gift from Christian Mosimann & Leonard Zon (Addgene plasmid # 68311 ; http://n2t.net/addgene:68311 ; RRID:Addgene_68311)
  • For your References section:

    Site-directed zebrafish transgenesis into single landing sites with the phiC31 integrase system. Mosimann C, Puller AC, Lawson KL, Tschopp P, Amsterdam A, Zon LI. Dev Dyn. 2013 Aug;242(8):949-63. doi: 10.1002/dvdy.23989. Epub 2013 Jul 3. 10.1002/dvdy.23989 PubMed 23723152