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PurposeNuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNot Available
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Vector typeWorm Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerab-3::NLS::GCaMP6s
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SpeciesC. elegans (nematode)
- Promoter rab-3
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer caggaaacagctatgac (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Prab-3::NLS::GCaMP6s was a gift from Andrew Leifer (Addgene plasmid # 68119 ; http://n2t.net/addgene:68119 ; RRID:Addgene_68119) -
For your References section:
Whole-brain calcium imaging with cellular resolution in freely behaving Caenorhabditis elegans. Nguyen JP, Shipley FB, Linder AN, Plummer GS, Liu M, Setru SU, Shaevitz JW, Leifer AM. Proc Natl Acad Sci U S A. 2015 Dec 28. pii: 201507110. 10.1073/pnas.1507110112 PubMed 26712014
Map uploaded by the depositor.
