Skip to main content
Addgene

ERmoxGFP
(Plasmid #68072)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 68072 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    ER-mRFP
  • Backbone manufacturer
    Snapp et al. PMID 16617114, Addgene plasmid 62236
  • Modifications to backbone
    ER-RFP contains fusions of the bovine prolactin signal sequence, mRFP, and a KDEL ER retention sequence. mRFP was replaced with moxGFP to create ERmoxGFP.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    moxGFP
  • Species
    Synthetic
  • Promoter CMV
  • Tags / Fusion Proteins
    • bovine prolactin signal sequence (N terminal on backbone)
    • KDEL ER retention sequence (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ERmoxGFP was a gift from Erik Snapp (Addgene plasmid # 68072 ; http://n2t.net/addgene:68072 ; RRID:Addgene_68072)

  • For your References section:

    A palette of fluorescent proteins optimized for diverse cellular environments. Costantini LM, Baloban M, Markwardt ML, Rizzo M, Guo F, Verkhusha VV, Snapp EL. Nat Commun. 2015 Jul 9;6:7670. doi: 10.1038/ncomms8670. 10.1038/ncomms8670 PubMed 26158227
Related items:
Commonly requested with: