pABD1031
(Plasmid #68004)

• Purpose: Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
• Depositing Lab: Bruce Downie
• Publication: Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):E4120-E4129. doi: 10.1073/pnas.1711919115. Epub 2018 Apr 9.

Backbone

• Vector backbone: pSITE-BiFC-C1cec
• Backbone manufacturer: Martin et al 2009 The Plant Journal, 59 150-162 http://onlinelibrary.wiley.com/doi/10.1111/j.1365-313X.2009.03850.x
• Backbone size w/o insert (bp): 8000
• Total vector size (bp): 9439
• Vector type: Plant Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin and Streptomycin, 20 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PHY-INTERACTING FACTOR 1 (PIF1)
• Alt name: PHYTOCHROME INTERACTING FACTOR 3-LIKE 5, PIL5
• Insert Size (bp): 1439
• GenBank ID: AT2G20180
• Entrez Gene: PIL5 (a.k.a. AT2G20180, PHY-INTERACTING FACTOR 1, PIF1, T2G17.2, T2G17_2, phytochrome interacting factor 3-like 5)
• Promoter: 2X Cauliflower Mosaic Virus 35S promoter
• Tag / Fusion Protein:
  • eYFP (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry