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PB-EF1-IRES-NEO deleted ITR
(Plasmid #67906)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67906 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB-EF1-IRES-NEO
  • Total vector size (bp) 6892
  • Modifications to backbone
    Deleted ITRs TTAACCCTAGAAAGATAATCATATTGTGACGTACGTTAAAGATAATCATGTGTAAAATTGACGCATG and CATGCGTCAATTTTACGCAGACTATCTTTCTAGGGTTAA
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NeoR
  • Species
    Synthetic
  • Entrez Gene
    neoR (a.k.a. pSH111_227_223)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer GGGTTCCGCGCACATTTC
  • 3′ sequencing primer CAGTCATCCTCGGCAAACTCTTT
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-EF1-IRES-NEO deleted ITR was a gift from Alex Kentsis (Addgene plasmid # 67906 ; http://n2t.net/addgene:67906 ; RRID:Addgene_67906)

  • For your References section:

    Genomic DNA transposition induced by human PGBD5. Henssen AG, Henaff E, Jiang E, Eisenberg AR, Carson JR, Villasante CM, Ray M, Still E, Burns M, Gandara J, Feschotte C, Mason CE, Kentsis A. Elife. 2015 Sep 25;4. pii: e10565. doi: 10.7554/eLife.10565. 10.7554/eLife.10565 PubMed 26406119
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