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Addgene

p4XBRE-SBE-SV40-GL3
(Plasmid #67811)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67811 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Id1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Id1 (a.k.a. AI323524, D2Wsu140e, Idb1, bHLHb24)
  • Promoter Id1/SV40
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer RVprimer3
  • 3′ sequencing primer LucNRev
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p4XBRE-SBE-SV40-GL3 was a gift from Ron Prywes (Addgene plasmid # 67811 ; http://n2t.net/addgene:67811 ; RRID:Addgene_67811)

  • For your References section:

    Serum regulation of Id1 expression by a BMP pathway and BMP responsive element. Lewis TC, Prywes R. Biochim Biophys Acta. 2013 Oct;1829(10):1147-59. doi: 10.1016/j.bbagrm.2013.08.002. Epub 2013 Aug 13. 10.1016/j.bbagrm.2013.08.002 PubMed 23948603
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