pCMV-HA-Ebp1
(Plasmid
#67792)
-
PurposeMammalian expression of Ebp1 tagged with HA
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67792 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepCMV
-
Backbone manufacturerClontech
- Backbone size w/o insert (bp) 3800
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameEbp1
-
SpeciesH. sapiens (human)
-
Entrez GenePA2G4 (a.k.a. EBP1, HG4-1, ITAF45, p38-2G4)
- Promoter CMV
-
Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCMV-HA-Ebp1 was a gift from Stephen Goff (Addgene plasmid # 67792 ; http://n2t.net/addgene:67792 ; RRID:Addgene_67792) -
For your References section:
EBP1, a novel host factor involved in primer binding site-dependent restriction of moloney murine leukemia virus in embryonic cells. Wang GZ, Wolf D, Goff SP. J Virol. 2014 Feb;88(3):1825-9. doi: 10.1128/JVI.02578-13. Epub 2013 Nov 13. 10.1128/JVI.02578-13 PubMed 24227866