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pGEX-4T-1-Shp2 T7DA/S189D SH2s
(Plasmid #67577)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67577 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-1
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Src homology 2 domain-containing phosphatase 2
  • Alt name
    Shp2
  • Species
    M. musculus (mouse)
  • Mutation
    Deleted amino acid residues 219-597, changed T73 and S189 to D
  • Tag / Fusion Protein
    • GST (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-4T-1-Shp2 T7DA/S189D SH2s was a gift from Graeme Carnegie (Addgene plasmid # 67577 ; http://n2t.net/addgene:67577 ; RRID:Addgene_67577)

  • For your References section:

    Protein Kinase A (PKA) Phosphorylation of Shp2 Protein Inhibits Its Phosphatase Activity and Modulates Ligand Specificity. Burmeister BT, Wang L, Gold MG, Skidgel RA, O'Bryan JP, Carnegie GK. J Biol Chem. 2015 May 8;290(19):12058-67. doi: 10.1074/jbc.M115.642983. Epub 2015 Mar 23. 10.1074/jbc.M115.642983 PubMed 25802336
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