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Addgene

pEGFP-FLAG-AKAP-Lbc
(Plasmid #67571)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67571 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    A-kinase anchoring protein Lbc
  • Alt name
    AKAP-Lbc
  • Alt name
    AKAP13
  • Species
    H. sapiens (human)
  • Mutation
    R2604G in AKAP
  • GenBank ID
    NM_006738.5
  • Entrez Gene
    AKAP13 (a.k.a. AKAP-13, AKAP-Lbc, ARHGEF13, BRX, HA-3, Ht31, LBC, PRKA13, PROTO-LB, PROTO-LBC, c-lbc, p47)
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • GFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-FLAG-AKAP-Lbc was a gift from Graeme Carnegie (Addgene plasmid # 67571 ; http://n2t.net/addgene:67571 ; RRID:Addgene_67571)

  • For your References section:

    Protein Kinase A (PKA) Phosphorylation of Shp2 Protein Inhibits Its Phosphatase Activity and Modulates Ligand Specificity. Burmeister BT, Wang L, Gold MG, Skidgel RA, O'Bryan JP, Carnegie GK. J Biol Chem. 2015 May 8;290(19):12058-67. doi: 10.1074/jbc.M115.642983. Epub 2015 Mar 23. 10.1074/jbc.M115.642983 PubMed 25802336
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