LZRS ATG12 ires zeo
(Plasmid
#67531)
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PurposeFor retroviral based stable expression of human ATG12
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 67531 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneLZRS ires zeocin
- Backbone size w/o insert (bp) 13200
- Total vector size (bp) 13600
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Vector typeMammalian Expression, Retroviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAPG12
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Alt nameATG12
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SpeciesH. sapiens (human)
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Entrez GeneATG12 (a.k.a. APG12, APG12L, FBR93, HAPG12)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer LZRS (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
LZRS ATG12 ires zeo was a gift from Stephen Tait (Addgene plasmid # 67531 ; http://n2t.net/addgene:67531 ; RRID:Addgene_67531) -
For your References section:
Ubiquitination and proteasomal degradation of ATG12 regulates its proapoptotic activity. Haller M, Hock AK, Giampazolias E, Oberst A, Green DR, Debnath J, Ryan KM, Vousden KH, Tait SW. Autophagy. 2014;10(12):2269-78. doi: 10.4161/15548627.2014.981914. 10.4161/15548627.2014.981914 PubMed 25629932