pCMV3-SMAC-HA-eGFP
(Plasmid #67489)

• Purpose: Targets HA-eGFP to mitochondrial intermembrane space (IMS)
• Depositing Lab: Richard Kahn
• Publication: Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523. doi: 10.1080/21592799.2015.1090523. eCollection 2015 Jul-Sep.

Backbone

• Vector backbone: pCMV(delta3)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMAC
• Alt name: pRK111
• Species: H. sapiens (human)
• Mutation: aa1-59
• Entrez Gene: DIABLO (a.k.a. DFNA64, SMAC)
• Promoter: attenuated CMV
• Tag / Fusion Protein:
  • HA-eGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Additional References: (1) For pCMV0-6 vectors: Morita, et al (2012) Biotechniques (PMCID: 3759224); (2) For targeting proteins to the IMS using the leader sequence from SMAC/Diablo: Sabharwal, et al (2013) Biochem J 456: 337-46, and (3) Ozawa, et al (2007) ACS Chemical Biology 2: 176-186.