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pcDNA3.1_iSH2-pMag(3x)-iRFP
(Plasmid #67304)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67304 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5354
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    iSH2-pMag(3x)-iRFP
  • Alt name
    inter-SH2 (iSH2) domain derived from a ​p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3K).
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    3096
  • Entrez Gene
    PIK3R1 (a.k.a. AGM7, GRB1, IMD36, p85, p85-ALPHA, p85alpha)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_iSH2-pMag(3x)-iRFP was a gift from Moritoshi Sato (Addgene plasmid # 67304 ; http://n2t.net/addgene:67304 ; RRID:Addgene_67304)

  • For your References section:

    Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Kawano F, Suzuki H, Furuya A, Sato M. Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256. 10.1038/ncomms7256 PubMed 25708714
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