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PurposeFluorescent indicator for PI(3,4,5)P3
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67301 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5348
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAktPH-mCherry
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Alt namepleckstrin homology (PH) domain derived from Akt1
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)2042
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Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1_AktPH-mCherry was a gift from Moritoshi Sato (Addgene plasmid # 67301 ; http://n2t.net/addgene:67301 ; RRID:Addgene_67301) -
For your References section:
Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Kawano F, Suzuki H, Furuya A, Sato M. Nat Commun. 2015 Feb 24;6:6256. doi: 10.1038/ncomms7256. 10.1038/ncomms7256 PubMed 25708714