p64PLA5
(Plasmid #67284)

• Purpose: 6/4 photolyase gene from Drosophila in pFastbac vector, intermediate step in insect cell expression
• Depositing Lab: Aziz Sancar
• Publication: Selby et al Biochemistry. 2012 Jan 10;51(1):167-71. doi: 10.1021/bi201536w. Epub 2011 Dec 27.

Backbone

• Vector backbone: pFastbac
• Backbone manufacturer: Gibco BRL
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6300
• Modifications to backbone: yes
• Vector type: Intermediate for insect expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 6/4 photolyase from Drosophila
• Species: D. melanogaster (fly)
• Insert Size (bp): 1632
• Mutation: none known
• GenBank ID: NT_003779.5
• Tag / Fusion Protein:
  • Flag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: Polyhedrin-F
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry