pfbiRS
(Plasmid
#67283)
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PurposeExpresses the fbiC gene from Mycobacterium smegmatis
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 67283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRSFDuet-1
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 3829
- Total vector size (bp) 6500
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsUse strain BL21 for induction/expression
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Copy numberUnknown
Gene/Insert
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Gene/Insert namefbiC gene from Mycobacterium smegmatis
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Alt nameFo synthase
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SpeciesMycobacterium smegmatis
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Insert Size (bp)2700
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Mutationnon known
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GenBank IDNC_008596.1
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Tag
/ Fusion Protein
- polyhistidine (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer LacI-R
- 3′ sequencing primer DuetUP2 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gene is induced with IPTG. Following induction, Fo present in media.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pfbiRS was a gift from Aziz Sancar (Addgene plasmid # 67283 ; http://n2t.net/addgene:67283 ; RRID:Addgene_67283) -
For your References section:
The second chromophore in Drosophila photolyase/cryptochrome family photoreceptors. Selby CP, Sancar A. Biochemistry. 2012 Jan 10;51(1):167-71. doi: 10.1021/bi201536w. Epub 2011 Dec 27. 10.1021/bi201536w PubMed 22175817