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Addgene

pfbiRS
(Plasmid #67283)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 67283 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRSFDuet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3829
  • Total vector size (bp) 6500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use strain BL21 for induction/expression
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    fbiC gene from Mycobacterium smegmatis
  • Alt name
    Fo synthase
  • Species
    Mycobacterium smegmatis
  • Insert Size (bp)
    2700
  • Mutation
    non known
  • GenBank ID
    NC_008596.1
  • Tag / Fusion Protein
    • polyhistidine (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer LacI-R
  • 3′ sequencing primer DuetUP2
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene is induced with IPTG. Following induction, Fo present in media.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pfbiRS was a gift from Aziz Sancar (Addgene plasmid # 67283 ; http://n2t.net/addgene:67283 ; RRID:Addgene_67283)
  • For your References section:

    The second chromophore in Drosophila photolyase/cryptochrome family photoreceptors. Selby CP, Sancar A. Biochemistry. 2012 Jan 10;51(1):167-71. doi: 10.1021/bi201536w. Epub 2011 Dec 27. 10.1021/bi201536w PubMed 22175817
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