pMalAnPL
(Plasmid #67281)

• Purpose: Expresses MBP-Anacystis nidulans photolyase in E. coli
• Depositing Lab: Aziz Sancar
• Publication: A. nidulans photolyase-MBP fusion protein (unpublished)

Backbone

• Vector backbone: pMalc2
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 6700
• Total vector size (bp): 8000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: photolyase Anacystis nidulans
• Species: Anacystis nidulans
• Insert Size (bp): 1454
• Mutation: none known
• GenBank ID:
• Tag / Fusion Protein:
  • maltose binding protein (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: MBP-F
• 3′ sequencing primer: pTrcHis-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry